Improved Host Range Selection for Recombinant Modified Vaccinia Virus Ankara
نویسندگان
چکیده
منابع مشابه
Transient host range selection for genetic engineering of modified vaccinia virus Ankara.
Recombinant vaccinia viruses are extremely valuable tools for research in molecular biology and immunology. The extension of vaccinia vector technology to replication-deficient and safety-tested virus strains such as modified vaccinia virus Ankara (MVA) have made this versatile eukaryotic expression system even more attractive for basic and clinical research. Here, we report on easily obtaining...
متن کاملGenomic sequence of chorioallantois vaccinia virus Ankara, the ancestor of modified vaccinia virus Ankara.
Chorioallantois vaccinia virus Ankara (CVA) is the parental virus of modified vaccinia virus Ankara (MVA), which was derived from CVA by more than 570 passages in chicken embryo fibroblasts (CEF). MVA became severely host-cell-restricted to avian cells and has strongly diminished virulence in mammalian hosts, while maintaining good immunogenicity. We determined the complete coding sequence of t...
متن کاملGeneration and evaluation of a recombinant modified vaccinia virus Ankara vaccine for rabies.
Modified vaccinia virus Ankara (MVA) has become a vaccine vector of choice for recombinant vaccine development. A MVA-based rabies vaccine would be advantageous for use as a vaccine for dogs (and wildlife), particularly if it proves innocuous and efficacious by the oral route. Here, the generation and immunological testing of a recombinant MVA expressing a rabies virus glycoprotein gene is desc...
متن کاملProtective Efficacy of Recombinant Modified Vaccinia Virus Ankara Delivering Middle East Respiratory Syndrome Coronavirus Spike Glycoprotein.
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans. We tested a recombinant modified vaccinia virus Ankara (MVA) vaccine expressing full-length MERS-CoV spike (S) glycoprotein by immunizing BALB/c mice with either intramuscular or subcutaneous regimens. In all cases, MVA-MERS-S induced MERS-CoV-specific CD8(+) T cells and virus-neutralizing antib...
متن کاملPresentation of Recombinant Proteins in Modified Vaccinia Virus Ankara Extracellular Enveloped Virions
Modified vaccinia virus Ankara (MVA) is a highly attenuated vaccinia virus strain developed during the smallpox eradication campaign. Nowadays recombinant attenuated poxviruses gain importance as live carrier vaccines against different infectious diseases and in cancer therapy. The aim of this work was to develop recombinant viral vectors, for presentation of a foreign antigen on the surface of...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2003
ISSN: 0736-6205,1940-9818
DOI: 10.2144/03344bm02